cloning vector การใช้

• Cloning vectors in yeast include yeast artificial chromosomes ( YACs ).
• They are often used as a cloning vector in genetic engineering.
• One bacterial plasmid used in genetic engineering as a plasmid cloning vector is pUC18.
• Genome size varies among different organisms and the cloning vector must be selected accordingly.
• P1 was developed as a cloning vector by Nat Sternberg and colleagues in the 1990s.
• A plasmid cloning vector is typically used to clone DNA fragments of up to 15 kbp.
• This method requires that restriction maps of the cloning vector and the insert are already available.
• His cloning vectors were also used to develop the method for oligonucleotide site-directed mutagenesis.
• There are many types of cloning vectors, but the most commonly used ones are genetically engineered plasmids.
• Formation of recombinant DNA requires a cloning vector, a DNA molecule that replicates within a living cell.
• The probe protein is often produced in " E . coli " using an expression cloning vector.
• In cases where DNA fragments are cloned before sequencing, the resulting sequence may contain parts of the cloning vector.
• In contrast, PCR-based cloning and next-generation sequencing technologies based on pyrosequencing often avoid using cloning vectors.
• The cloning vector is treated with a restriction endonuclease to cleave the DNA at the site where foreign DNA will be inserted.
• Modern cloning vectors include selectable antibiotic resistance markers, which allow only cells in which the vector has been transfected, to grow.
• It has two antibiotic resistance genes, and a number of convenient unique restriction sites that made it suitable as a cloning vector.
• Other cloning vectors may use topoisomerase instead of ligase and cloning may be done more rapidly without the need for restriction digest of the vector or insert.
• Naturally occurring circular plasmids can be modified to contain multiple resistance genes and several unique restriction sites, making them valuable tools as cloning vectors in biotechnology applications.
• The primers may have additional sequences added to their 5'ends, such as sequences for restriction enzyme recognition sites needed for ligation into a cloning vector.